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[Content determination] According to HPLC determination.
Chromatographic conditions and system suitability test: Use ODS as filler; use acetonitrile-water (20:80) (Adjust pH to 3 with phosphoric acid) as mobile phase; detection wavelength as 283nm. The number of theoretical plates calculated based on the naringin peak should not be less than 3000.
Preparation of reference solution: Take an appropriate amount of naringin reference substance and neohesperidin reference substance, weigh them accurately, then add methanol to make a solution containing 80ug each of naringin and neohesperidin per 1ml.
Preparation of test solution: Take about 0.2g coarse powder, weigh it accurately, place it in a stoppered conical flask, accurately add 50ml of methanol, weigh it, heat to reflux for 1.5 hours, let it cool, then weigh it. Use methanol to make up the lost weight, shake well, strain. Accurately measure 10ml of the continuous filtrate, put it in a 25ml measuring flask, add methanol to the mark, shake well, and get it.
Detection method: Precisely draw 10ul each of the reference solution and test solution, and inject it into the HPLC instrument for determination.
This product is calculated as a dry product, and contains naringin (C27H32O14) not less than 4.0%, and neohesperidin (C28H34O15) not less than 3.0.
